Work out absorbance to concentration instantly with clear inputs, formula shown and shareable results.
Reading a concentration off a spectrophotometer inverts Beer-Lambert: c = A/(epsilon x l). An absorbance of 0.75 in a 1 cm cell with molar absorptivity 15,000 corresponds to 5.0e-5 mol/L, or 50 micromolar. Accuracy is best between absorbance 0.1 and 1.0, so the calculator flags readings outside that band.
Inverted Beer-Lambert
c = A / (epsilon x l)
Transmittance check
%T = 100 x 10^-A
Build a calibration line from standards instead: the slope is epsilon times the path length, so the unknown reads straight off the graph.
Yes. A 10 cm cell multiplies absorbance tenfold, which is a standard trick for trace analysis.