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Defines one unit as one micromole of product per minute and divides by protein mass to give specific activity.
Enzyme units
1 U = 1 micromol of product per minute; specific activity = U / mg protein; 1 katal = 1 mol/s, so 1 U = 16.67 nkat
It tracks purification: total units fall while specific activity rises as contaminating protein is removed.
Yes. Activity is defined from the initial linear phase before substrate depletion or product inhibition.