Calculate GC content of a sequence and the melting temperature it implies.
GC content determines duplex stability because GC pairs have three hydrogen bonds to AT’s two. It also predicts melting temperature and correlates with genome organisation across organisms. GC-rich templates form secondary structures that resist amplification, which is why they often require DMSO or betaine additives.
GC Content
GC % = (G + C) ÷ total bases × 100
GC % = (G + C) ÷ total bases × 100 GC content determines duplex stability because GC pairs have three hydrogen bonds to AT’s two. It also predicts melting temperature and correlates with genome organisation across organisms.
GC-rich templates form secondary structures that resist amplification, which is why they often require DMSO or betaine additives.
This calculator takes 2 inputs: Number of G and C bases, Total sequence length. The pre-filled defaults are a realistic starting point — replace them with figures from your own environment for a result you can act on.