Calculate viral volume required for a target multiplicity of infection.
Because virions distribute randomly, an MOI of 1 leaves about 37 per cent of cells uninfected rather than infecting all of them. Reaching near-complete infection requires an MOI of around five or more. Assuming MOI of 1 infects every cell is the most common transduction planning error, and the Poisson figure is what corrects it.
MOI
Volume = (cells × MOI) ÷ titre; uninfected fraction = e^(−MOI)
Volume = (cells × MOI) ÷ titre; uninfected fraction = e^(−MOI) Because virions distribute randomly, an MOI of 1 leaves about 37 per cent of cells uninfected rather than infecting all of them. Reaching near-complete infection requires an MOI of around five or more.
Assuming MOI of 1 infects every cell is the most common transduction planning error, and the Poisson figure is what corrects it.
This calculator takes 4 inputs: Cells per well, Target MOI, Viral titre, Number of wells. The pre-filled defaults are a realistic starting point — replace them with figures from your own environment for a result you can act on.