Work out pcr cycle yield instantly with clear inputs, formula shown and shareable results.
Compounds the amplification efficiency across the cycle count, the exponential model behind PCR yield.
PCR yield
Copies = starting copies x (1 + efficiency)^cycles; perfect efficiency doubles product each cycle
Reagent depletion, polymerase inactivation and product reannealing end the exponential phase after 25 to 35 cycles.
90 to 110% is the accepted window for quantitative PCR assays.