Calculate reagent volumes for a PCR master mix across multiple reactions.
Each component volume follows C₁V₁ = C₂V₂ scaled by the number of reactions. Preparing about 10 per cent excess matters because pipetting losses otherwise leave the final reactions short. A master mix reduces both pipetting error and setup time, and it is the single most effective way to improve reproducibility between replicate reactions.
PCR Mix
Component volume = (final concentration × reaction volume) ÷ stock concentration, × reactions
Component volume = (final concentration × reaction volume) ÷ stock concentration, × reactions Each component volume follows C₁V₁ = C₂V₂ scaled by the number of reactions. Preparing about 10 per cent excess matters because pipetting losses otherwise leave the final reactions short.
A master mix reduces both pipetting error and setup time, and it is the single most effective way to improve reproducibility between replicate reactions.
This calculator takes 6 inputs: Number of reactions, Volume per reaction, Excess to allow for pipetting loss, Primer stock concentration, Final primer concentration, Template per reaction. The pre-filled defaults are a realistic starting point — replace them with figures from your own environment for a result you can act on.