Work out qpcr efficiency instantly with clear inputs, formula shown and shareable results.
Converts a standard curve slope into amplification efficiency, where a slope of minus 3.32 corresponds to perfect doubling.
qPCR efficiency
Amplification = 10^(-1/slope); efficiency (%) = (amplification - 1) x 100; a slope of -3.322 gives 100%
It is minus log10(2) inverted: a tenfold dilution shifts Ct by 3.32 cycles when each cycle doubles the product.
Efficiency above 100%, usually caused by inhibitors, pipetting error or primer dimers rather than genuine over-amplification.