Calculate the molecular weight of an RNA molecule and convert mass to moles.
RNA nucleotides average 321.5 daltons, marginally different from DNA because ribose carries an additional hydroxyl. The mass-to-mole conversion is essential for setting up in vitro transcription and transfection. Molar amount rather than mass determines how many transcripts are delivered, which is what actually drives expression level.
RNA Molecular Weight
MW ≈ 321.5 × nucleotides + 159 for single-stranded RNA
MW ≈ 321.5 × nucleotides + 159 for single-stranded RNA RNA nucleotides average 321.5 daltons, marginally different from DNA because ribose carries an additional hydroxyl. The mass-to-mole conversion is essential for setting up in vitro transcription and transfection.
Molar amount rather than mass determines how many transcripts are delivered, which is what actually drives expression level.
This calculator takes 3 inputs: Length in nucleotides, Quantity available, Average mass per nucleotide. The pre-filled defaults are a realistic starting point — replace them with figures from your own environment for a result you can act on.