Work out chlorophyll concentration instantly with clear inputs, formula shown and shareable results.
Applies Arnon's equations for chlorophyll in 80 percent acetone and scales the result to milligrams per gram of fresh tissue.
Arnon equations
Chl a = 12.7 A663 - 2.69 A645; Chl b = 22.9 A645 - 4.68 A663; total = 20.2 A645 + 8.02 A663, all in mg/L
80% acetone. Different coefficients apply for methanol, ethanol or DMSO extracts.
It typically runs about 3:1 in sun leaves and falls in shade leaves, which invest more in light-harvesting complexes.