Work out dna concentration instantly with clear inputs, formula shown and shareable results.
Applies the convention that one absorbance unit at 260 nm equals 50 micrograms per millilitre of double-stranded DNA, and checks purity with the A260/A280 ratio.
Nucleic acid quantification
Concentration (microg/mL) = A260 x 50 x dilution factor for double-stranded DNA (40 for RNA, 33 for single-stranded DNA)
Pure DNA absorbs about 1.8 times as strongly at 260 nm as at 280 nm; protein absorbs strongly at 280 nm and lowers the ratio.
It detects guanidine and other buffer contaminants that absorb near 230 nm.